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分类抗体
级别优级纯GR
产品规格SIGMA E4127-0.1MG
CAS62229-50-9
用途范围生物科研
保存温度2-8℃
英文名称Epidermal Growth Factor from murine submaxillary g
供应商 上海极威生物科技有限公司
· 产品编号:E4127
· 中文名称:表皮生长因子 来源于小鼠颌下腺
· 英文名称:Epidermal Growth Factor from murine submaxillary gland
· 英文别名:EGF Epidermal cell growth factor
· CAS号:62229-50-9
· 保存温度:2-8 °C
· EC号:263-468-7
· MDL号:MFCD01634807
产品规格价格(RMB)
· E4127-0.1MG1,838.07元
· E4127-5X0.1MG7,376.85元
The biological activity is measured in a proliferation assay using BALB/MK cells.
Qualified for use as a mitogen for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.
Purified by gel filtration.
Lyophilized from 0.2 μm filtered solution in ammonium acetate buffer.
Epidermal Growth Factor (EGF) is a 6 kDa polypeptide discovered by Cohen and Levi -Montalcini and isolated from mouse submaxillary glands. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells. In tissue cultures EGF can act to reduce or eliminate the requirement for serum and is often used in conjunction with other media additives and hormones, such as Insulin (Product No. I1882), Transferrin (Product No. T 5391), and Prostaglandin E1 (Product No. P7527). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins.
Human EGF was originally isolated from human urine as β-Urogastrone, an inhibitor of gastric acid secretion. Mouse and human EGF are 70% homologous in their sequence of 53 amino acids, act upon the same EGF receptor, elicit nearly identical biological effects and are highly inter-species cross-reactive.
EGF is structurally homologous to human Transforming Growth Factor-α (Product Nos. T7533 and T2035), which exerts its actions through EGF receptors. EGF is also homologous to a sequence contained in a 19 kDa protein of Vaccinia virus, which appears to utilize the EGF receptor to gain entry into cells. The EGF receptor is a 170 kDa glycoprotein having EGF-activated protein tyrosine kinase activity. Platelet-Derived Growth Factor(PDGF, Product No. P8147) transmodulates the EGF receptor by reduc ing both its EGF affinity and its kinase activity.
Related Categories | Cancer Research Cell Biology Cell Culture Cell Culture Supplies Cell Signaling and Neuroscience更多... |
form | lyophilized powder |
potency | 0.1-10 ng/mL ED50/EC50 |
total impurities | endotoxin, tested |
suitability | suitable for cell culture |
storage temp. | 2-8°C |
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更新时间:2024/3/6 18:04:39
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